• Cardiac cycle, of the heart
• Contraction of the muscles of the heart, or a perceived effect of it, such as:
  • Heart sounds, the noises generated by the beating heart and the resultant flow of blood through it
  • Pulse, the beat caused by the pumping action of the heart
  • Heartbeat, contraction visualized on echocardiography

• Heartbeat (computing), a periodic signal to indicate normal operation or to synchronize parts of a system
• Heartbeat (program), clustering software from Linux-HA project
• Heartbeat network, a network of cluster nodes which monitors each node and facilitates communication

• Heartbeat (1938 film), a 1938 French comedy, remade in 1999 as Le schpountz
• Heartbeat (1946 film), a U.S. feature film by Sam Wood, with Ginger Rogers
• La Chamade (film) (English title: Heartbeat), a 1968 French feature film by Alain Cavalier
• Heart Beat (film), a 1980 U.S. feature film about the love triangle between Jack Kerouac, Carolyn Cassady and Neal Cassady
• Heart Beats (film), a 2007 Indian feature film in the Malayalam language
• Heartbeats (film), a 2010 Canadian French-English feature film by Xavier Dolan
• Heartbeat (2011 film), a South Korean feature film about the illegal trade in human organs
• Heartbeat (2012 film), an Austrian short film
• Heartbeat (2014 film), a Canadian film

• HeartBeat (1988 TV series), a 1988–89 American medical drama that aired on ABC
• Heartbeat (UK TV series), a 1992–2010 British police drama
• Heartbeat (1993 film), a 1993 NBC TV film
• Heartbeat (2016 TV series), a 2016 American medical drama that aired on NBC

Bands

• The Heartbeats, a 1950s American doo-wop group
• The Heart Beats, a 1960s all-female garage rock band

Albums

• Heartbeat (Bad Boys Blue album), 1986
• Heartbeat (Curtis Mayfield album), 1979
• Heartbeat (Da' T.R.U.T.H. album), 2014
• Heartbeat (Don Johnson album), 1986
• Heartbeat (G.E.M. album), 2015
• Heartbeat (Jasmine Rae album), 2015
• Heartbeat (Jeremy Rosado album), 2015
• Heartbeat (The Oak Ridge Boys album), 1987
• Heartbeat (Ruby Lin album), 1999
• Heartbeat (Ryuichi Sakamoto album), 1991
• Heartbeat (Sarah Engels album), 2011
• Heart Beat (Space Tribe album), 2002
• Heart Beat (Wang Leehom album), 2008
• Heart Beats (Dami Im album), 2014
• Heart Beats (Danny album), 2007
• Heart Beats (Keystone Trio album), 1995

Songs

• "Heartbeat" (2PM song), 2009
• "Heartbeat" (Annie song), 2004
• "Heartbeat" (Buddy Holly song), 1958
• "Heartbeat" (Can-linn song), 2014
• "Heartbeat" (Carrie Underwood song), 2015
• "Heartbeat" (Childish Gambino song), 2011
• "Heartbeat" (Don Johnson song), 1986
• "Heartbeat" (Enrique Iglesias song), 2010
• "Heartbeat" (The Fray song), 2011
• "Heartbeat" (Girlfriend song), 1993
• "Heartbeat" (Jimmy Somerville song), 1995
• "Heartbeat" (Justs song), 2016
• "Heartbeat" (King Crimson song), 1982
• "Heartbeat" (Late of the Pier song), 2008
• "Heartbeat", by Leona Lewis on her single "I Got You", 2009
• "Heartbeat", by Madonna on her album Hard Candy, 2008
• "Heartbeat" (Margaret song), 2015
• "Heartbeat" (Nina Sky song), 2012
• "Heartbeat", by Nneka, 2008
• "Heartbeat", by Red 7, 1985
• "Heartbeat" (Richard Orlinski and Eva Simons song), 2016
• "Heartbeat", by Ruby Murray, 1954
• "Heartbeat", by href="/wiki/Showaddywaddy" title="Showaddyw