Amrit Manthan is the story of two sisters Amrit and Nimrit. Starting off as best friends, fate leads them to turn into enemies. Nimrit survives Amrit's attempt to kill her but her face is ruined and changed by plastic surgery and she returns as Natasha Oberoi to seize everything that Amrit has taken from her. A lookalike of Nimrit (later revealed to be her long lost twin) comes to town named Shivangi who helps Nimrit in her battle against Amrit and succeeds. Shivani then marries Tej and has a daughter called JoJo.

After a series of events, Nimrit thinks that her husband, Agam and Amrit have died, but they had survived. Shivangi has left Tej and JoJo. Amrit once again returns to ruin her sister's and Agam's life. Later, Amrit wants Gurbani, Nimrit and Agam's daughter to rebel against Nimrit. The plan fails and later Amrit and Nimrit have put their dark past behind them and Amrit has realised her mistake. Bani adores her Amrit maasi and her mother.

Agam gets killed by a new character Angad/Karan due to a misunderstanding. Amrit and Tej decide to get married. Angad forcefully marries Nimrit but later Nimrit accepts him after the misunderstanding is cleared and the show end with a happy ending.

• Adaa Khan as Rajkumari Amrit Kaur Sodhi / Amrit Tej Malik / Rajjo.
• Ankita Sharma as Nimrit Agam Malik(after plastic surgery)/ Nimrit Angad Malik
• Dimple Jhangiani as Rajkumari Nimrit Kaur Sodhi / Nimrit Agam Malik / Shivangi Tej Malik
• Navi Bhangu as Agam Mahinder Malik / Agam Sehgal
• Wasim Mushtaq as Tej Malik
• Angad Hasija as Angad Malik / Karan Chabiara
• Karan Veer Mehra as Yug Mehra
• S.P Lalwani as Mahinder Malik
• Gaurav Nanda as Bhupinder Singh and Pritam Bakshi
• Ananya Agarwal as Gurbani Agam Malik / Bani
• Dhriti Mehta as Jyoti Tej Malik
• Deepak Sandhu as Vishal Sehgal
• Ananya Khare as Indu Chabiara