Thin layer chromatography

Thin-layer chromatography is performed on a sheet of glass, plastic, or aluminium foil, which is coated with a thin layer of adsorbent material, usually silica gel, aluminium oxide (alumina), or cellulose. This layer of adsorbent is known as the stationary phase.

After the sample has been applied on the plate, a solvent or solvent mixture (known as the mobile phase) is drawn up the plate via capillary action. Because different analytes ascend the TLC plate at different rates, separation is achieved.

It is thus based on the principle of adsorption chromatography or partition chromatography or combination of both, depending on adsorbent, its treatment and nature of solvents employed. The components with more affinity towards stationary phase travels slower. Components with less affinity towards stationary phase travels faster.

Once separation occurs, the individual components are visualized as spots at a respective level of travel on the plate. Their nature or character is identified by means of suitable detection techniques.

If the substance is more polar, Rf value getting more than that of less polar material. Chromatography is adsorpyion and partition , while mixture of substance is allowed to pass through stationary and mobile phase , a compound become separate according to their Rf

the movement occur in such a way that the compound which have a higher affinity to the stationary phase , move slowly while the other compounds travel fast. Therefore, the separation of the mixture is attained

Editor: Ankita Added on: 2021-03-03 13:30:32 Total View:313