streak plate and spread plate

Aim : Standard plate count method to check viable cells 

Principle: There are microbiology techniques which helps us to count and check viability of microbes present in sample.sample are collected from air , water and soil .

These methods are :

Spread plate method : this method uses a L shaped loop made up of glass or metal with smooth surface for spreading microbes from sample over a plate which tells us about number of microbial colony in sample from which inoculation was taken .

Pour plate : This technique uses molten nutrient agar for growth of mixed culture in the agar . Heat sensitivity bacterium growth are reduced in this method as agar is hot and can effect growth of heat sensitive and obligate anaerobes.

Sterak plate methods:This technique involves T-steak and Quadrant method

This method of bacterial culture involves continuous striking to get separated colonies of microbes from mixed samples . Individual colonies are isolated in this technique.

Observation:different platting methods are used to isolate colonies from mixed culture and used to check viability of colonies microbes.colonies of pure culture are isolated.

Result:different pure colonies were isolated and vibility can be checked .

Learning outcomes:spread plate method tells about no of colonies culture characteristics are also known .

Pour plate:used to check contamination in food stuffs and helpful to calculate microbes/mL in a sample.

Streak plate is commonly and widely used bacterial culture technique for isolating pure colonies of microbe