kirby bauer method

Kirby Bauer Method Test:

•      Prepared the MHA ( Mueller-Hinton agar )media, put it into petri dish and  allow  it for solidification .

•      After solidification swabbed a Mueller-Hinton agar plate with one type of the bacterial species .

•      Dipped a sterile swab into the broth and express any excess moisture by pressing the swab against the side of the tube.

•      Swabbed the surface of the agar plate completely and covered the bacteria over the entire agar.

•      After completed swabbing the plate, turn it 90 degrees and repeat the swabbing process. 

•      Run the swab around the circumference of the plate before discarding it in the discard bag.

•      Allow the surface to dry for about 5 minutes before placing antibiotic disks on the agar.

THE ANTIBIOTIC DISKS:

•      Used individual antibiotic dispensers and used a pair of sterile forceps to remove an antibiotic disc from the dispenser 

•      Placed the forceps in alcohol, flame the forceps until they catch on fire, let the flame go out sterile forceps.

•      Lightly touch each disc with sterile inoculating loop to make sure that it is in good contact with the agar surface and incubated at 37^o C

•      Make sure that all the instruments used in antibiotic testing should  be  properly sterilised  prior to use.