Isolation of DNA from blood

Isolation of DNA from Blood.

DNA was first isolated by Friedrich Miescher who discovered a

  substance called "nuclein" in 1869. Blood is the main source of DNA for genotype-

 related studies in humans. A rapid, efficient, and cost effective method for the

 isolation of genomic DNA from whole blood is needed for screening a large

 number of samples. There are many published protocols. Salting out method is

 one of the simplest of all the published methods. Following this procedure, it takes anywhere from 3 to 4 hrs to isolate DNA for large number of samples with

 the yield ranging (6 to10ug ) good quality of DNA from 300μL whole blood.

Blood collected in EDTA-containing vacationer tubes. As will all body fluids, blood represents a potential biohazard, thus care should be taken in all steps requiring handling of blood. If the subject is from a known high-risk category, additional precautions may be required. Blood samples stored at room temperature for DNA extraction within the same working day & also at refrigerator for later uses.