Chromatography-HPLC

HPLC high performance liquid chromatography 

• The process involves the interaction of the compounds in the analyte (which travels along with a mobile phase) across an immobile surface (stationary phase).


• The compounds bind at specific regions of the stationary phase based on certain physical and chemical properties. These bound molecules are then eluted with a suitable buffer and the same are collected with time.

These are:

ü Polarity

ü Charge

ü Molecular weight

ü Present of functional group

Application

can be used in both qualitative and quantitative applications that are for both compound quantification and identification. 

Normal phase HPLC is rarely used now, almost all HPLC separation can be performed in reverse phase. 

Reverse phase HPLC (RPLC) is ineffective for only a few separation types. HPLC is applied for molecular weight determination, in analytical chemistry, pharmaceutical and drug science, clinical sciences, food technology, and consumer products, combinatorial chemistry, polymer chemistry, environmental chemistry and green chemistry.