Biochemical test for microbial identification

To perform biochemical tests for microbial identification.

Theory:

Biochemical tests are the tests used for the identification of bacteria species based on the differences in the biochemical activities of different bacteria. Everything that a living organism does is the result of the activity of an ENZYME; the SUMMATION of the activities of all an organism's enzymes equals its BIOCHEMICAL FINGERPRINT. The types of biochemical reactions each organism undergoes act as a “thumbprint” for its identification. That is, by determining which set of enzymes are present in an unknown organism one can DESCRIBE & IDENTIFY that organism.

Assays for Metabolic Pathways:

Single substrate utilization

– Citrate utilization test.

– Acetate utilization test.

– Nitrate Reduction Test.

– Carbohydrate fermentation.

– MRVP test.

– Lysine agar test.

(A) Citrate utilization test

Requirements

– Media: per 1000 mL, pH 6.9.

– NH4H2PO4 (1 g).

– K2HPO4 (1 g).

– NaCl (5 g).

– Sodium citrate (2 g).

– MgSO4 (0.2 g).

– Agar (15 g).

– Bromthymol blue (0.08 g)

Editor: Ankita Added on: 2021-03-10 13:21:15 Total View:392